Bioorganic & Medicinal Chemistry
Volume 23, Issue 1, 1 January 2015, Pages 80–88
Giacoppo S1, Galuppo M1, De Nicola GR2, Iori R2, Bramanti P1, Mazzon E3.
1IRCCS Centro Neurolesi ‘Bonino-Pulejo’, Via Provinciale Palermo, contrada Casazza, 98124 Messina, Italy.
2Consiglio per la Ricerca e la sperimentazione in Agricoltura, Centro di Ricerca per le Colture Industriali (CRA-CIN), Via di Corticella 133, 40128 Bologna, Italy.
3IRCCS Centro Neurolesi ‘Bonino-Pulejo’, Via Provinciale Palermo, contrada Casazza, 98124 Messina, Italy. Electronic address: emazzon.irccs@gmail.com.
Received 19 September 2014, Revised 11 November 2014, Accepted 14 November 2014, Available online 22 November 2014
4(α-l-Rhamnosyloxy)-benzyl isothiocyanate (glucomoringin isothiocyanate; GMG-ITC) is released from the precursor 4(α-l-rhamnosyloxy)-benzyl glucosinolate (glucomoringin; GMG) by myrosinase (β-thioglucoside glucohydrolase; E.C. 3.2.1.147) catalyzed hydrolysis. GMG is an uncommon member of the glucosinolate group as it presents a unique characteristic consisting in a second glycosidic residue within the side chain. It is a typical glucosinolate found in large amounts in the seeds of Moringa oleifera Lam., the most widely distributed plant of the Moringaceae family. GMG was purified from seed-cake of M. oleifera and was hydrolyzed by myrosinase at neutral pH in order to form the corresponding GMG-ITC. This bioactive phytochemical can play a key role in counteracting the inflammatory response connected to the oxidative-related mechanisms as well as in the control of the neuronal cell death process, preserving spinal cord tissues after injury in mice. Spinal cord trauma was induced in mice by the application of vascular clips (force of 24g) for 1 min., via four-level T5-T8 after laminectomy. In particular, the purpose of this study was to investigate the dynamic changes occurring in the spinal cord after ip treatment with bioactive GMG-ITC produced 15 min before use from myrosinase-catalyzed hydrolysis of GMG (10mg/kg body weight+5 μl Myr mouse/day). The following parameters, such as histological damage, distribution of reticular fibers in connective tissue, nuclear factor (NF)-κB translocation and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκB-α) degradation, expression of inducible Nitric Oxide Synthases (iNOS), as well as apoptosis, were evaluated. In conclusion, our results show a protective effect of bioactive GMG-ITC on the secondary damage, following spinal cord injury, through an antioxidant mechanism of neuroprotection. Therefore, the bioactive phytochemical GMG-ITC freshly produced before use by myrosinase-catalyzed hydrolysis of pure GMG, could prove to be useful in the treatment of spinal cord trauma.
Copyright © 2014 Elsevier Ltd. All rights reserved.
KEYWORDS:
4(α-l-Rhamnosyloxy)-benzyl isothiocyanate; Apoptosis; Enzyme myrosinase; Glucomoringin; Inflammation; Moringa oleifera; Oxidative stress; Spinal cord injury
Resource: http://www.sciencedirect.com/science/article/pii/S0968089614008098